Publications

2024

  • Arnoult, N., and T. R. Cech. Telomerase Misbehaves after a Breakup. Science 383, 702-03 (2024).
  • Hemphill, W.O., Steiner, H. R., Kominsky, J.R., Wuttke, D.S., Cech, T.R. Transcription factors ERα and Sox2 have differing multiphasic DNA and RNA binding mechanisms. bioRxiv 03.1101/2024.03.18.585577 (2024).

2023

  • Hemphill, W. O., Fenske, R., Gooding, A. R., & Cech, T. R. (2023). PRC2 direct transfer from G-quadruplex RNA to dsDNA has implications for RNA-binding chromatin modifiers. Proc Natl Acad Sci U S A, 120, e2220528120.
  • Hemphill, W. O., Voong, C. K., Fenske, R., Goodrich, J. A., & Cech, T. R. (2023). Multiple RNA- and DNA-binding proteins exhibit direct transfer of polynucleotides with implications for target-site search. Proc Natl Acad Sci U S A, 120, e2220537120.

  • Song, J., Gooding, A. R., Hemphill, W. O., Love, B. D., Robertson, A., Yao, L., Zon, L. I., North, T. E., Kasinath, V., & Cech, T. R.  Structural basis for inactivation of PRC2 by G-quadruplex RNA. Science, 381, 1331-1337 (2023).

  • Strader, L. C., Staller, M. V., Willis, A. E., Faulkner, G. J., Beggs, J. D., & Cech, T. R. (2023). The complexity of transferring genetic information. Mol Cell, 83, 320-323.

  • Jansson-Fritzberg, L. I., Sousa, C. I., Smallegan, M. J., Song, J. J., Gooding, A. R., Kasinath, V., Rinn, J. L., & Cech, T. R. DNMT1 inhibition by pUG-fold quadruplex RNA. RNA, 29, 346–360 (2023).

  • Long, Y., Hwang, T., Gooding, A. R., Goodrich, K. J., Vallery, T. K., Rinn, J. L., & Cech, T. R. Evaluation of the RNA-dependence of PRC2 binding to chromatin in human pluripotent stem cells. bioRxiv.  10.1101/2023.08.17.553776 (2023).

  • Forino, N. M., Woo, J. Z., Zaug, A. J., Jimenez, A. G., Cech, T. R., Rouskin, S., & Stone, M. D. Dissecting telomerase RNA structural heterogeneity in living human cells with DMS-MaPseq. bioRxiv.  10.1101/2023.10.04.560962, (2023).

2022

  • Jansson-Fritzberg, L. I., Sousa, C. I., Smallegan, M. J., Song, J. J., Gooding, A. R., Kasinath, V., Rinn, J. L., Cech, T. R. (2022). DNMT1 inhibition by pUG-fold quadruplex RNA. bioRxiv, doi:10.1101/2022.10.14.512272 (2022).
  • Kraft, K., Yost, K.E., Murphy, S.E., Magg, A., Long, Y., Corces, M.R., Granja, J.M., Wittler, L., Mundlos, S., Cech, T.R., Boettiger, A.N., Chang, H.Y. Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation. Proc Natl Acad Sci U S A 119, May 26 ePub (2022).
  • Zaug, A. J., Goodrich, K. J., Song, J. J., Sullivan, A. E. & Cech, T. R. Reconstitution of a telomeric replicon organized by CST. Nature, 608, 819-825 (2022).

2021

  • Agarwal, N., Rinaldetti, S., Cheikh, B. B., Zhou, Q., Hass, E. P., Jones, R. T., Joshi, M., LaBarbera, D. V., Knott, S. R. V., Cech, T. R., Theodorescu, D., TRIM28 is a transcriptional activator of the mutant TERT promoter in human bladder cancer. Proc Natl Acad Sci U S A, 118, e2102423118 (2021).
  • Zaug, A. J., Lim, C.J., Olson, C.L., Carilli, M.T., Goodrich, K.J., Wuttke, D.S., Cech, T.R., CST does not evict elongating telomerase but prevents initiation by ssDNA binding. Nucleic Acids Res 49, 11653-11665 (2021). 
  • Dumbovic, G., Braunschweig, U., Langner, H. K., Smallegan, M., Biayna, J., Hass, E. P., Jastrzebska, K., Blencowe, B., Cech, T. R., Caruthers, M. H., Rinn, J. L. Nuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention. Nat Commun, 12(1), 3308 (2021).
  • Lim, C. J., & Cech, T. R. Shaping human telomeres: from shelterin and CST complexes to telomeric chromatin organization. Nat Rev Mol Cell Biol, 22(4), 283-298 (2021).
  • Long, Y., & Cech, T. R. Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools. Methods, 191, 44-58 (2021).
  • Youmans, D. T., Gooding, A. R., Dowell, R. D., & Cech, T. R. Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. Mol Cell, 81(3), 488-501 e489 (2021).

2020

  • Stern, J. L., Hibshman, G., Hu, K., Ferrara, S. E., Costello, J. C., Kim, W., Tamayo,P., Cech, T.R., Huang, F. W. Mesenchymal and MAPK Expression Signatures Associate with Telomerase Promoter Mutations in Multiple Cancers. Mol Cancer Res, 18, 1050-1062 (2020).

  • Heenan, P. R., Wang, X., Gooding, A. R., Cech, T. R., & Perkins, T. T. Bending and looping of long DNA by Polycomb repressive complex 2 revealed by AFM imaging in liquid. Nucleic Acids Res, 48, 2969-2981 (2020).

  • Lim, C. J., Barbour, A. T., Zaug, A. J., Goodrich, K. J., McKay, A. E., Wuttke, D. S., & Cech, T. R. The structure of human CST reveals a decameric assembly bound to telomeric DNA. Science, 368, 1081-1085 (2020).

  • Long, Y., Hwang, T., Gooding, A. R., Goodrich, K. J., Rinn, J. L., & Cech, T. R.  RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells. Nat Genet, 52, 931-938 (2020).

  • Rowland, T.J., Bonham, A.J., Cech, T.R., Allele-specific proximal promoter hypomethylation of the telomerase reverse transcriptase gene associates with expression in multiple cancers. Mol Oncol, 14, 2358-2374 (2020).

2019

  • Rowland, T. J., Dumbovic, G., Hass, E. P., Rinn, J. L., & Cech, T. R. Single-cell imaging reveals unexpected heterogeneity of telomerase reverse transcriptase expression across human cancer cell lines. Proc Natl Acad Sci U S A, 116(37), 18488-18497. (2019).

  • Wang, X., Goodrich, K. J., Conlon, E. G., Gao, J., Erbse, A. H., Manley, J. L., & Cech, T. R. C9orf72 and triplet repeat disorder RNAs: G-quadruplex formation, binding to PRC2 and implications for disease mechanisms. RNA, 25(8), 935-947. (2019).

  • Wang, X., Long, Y., Paucek, R. D., Gooding, A. R., Lee, T., Burdorf, R. M., & Cech, T. R. Regulation of histone methylation by automethylation of PRC2. Genes Dev, 33(19-20), 1416-1427. (2019).

2018

  • Greenwood, J., Patel, H., Cech, T. R., & Cooper, J. P. Fission yeast telosomes: non-canonical histone-containing chromatin structures dependent on shelterin and RNA. Nucleic Acids Res.46, 8865-8875  (2018).

  • Schmidt, J. C., Zaug, A. J., Kufer, R., & Cech, T. R. Dynamics of human telomerase recruitment depend on template- telomere base pairing. Mol Biol Cell. 29, 869-880 (2018).

  • Shoffner, G. M., Wang, R., Podell, E., Cech, T. R., & Guo, F. In Crystallo Selection to Establish New RNA Crystal Contacts. Structure, 26, 1275-1283 (2018)

  • Youmans, D. T., Schmidt, J. C., & Cech, T. R. Live-cell imaging reveals the dynamics of PRC2 and recruitment to chromatin by SUZ12-associated subunits. Genes Dev, 32 794-805 (2018)

2017

  • 351.  Wang, X.,Goodrich, K.J.,Gooding, A.R., Naeem, H., Archer, S., Paucek, R.D., Youmans, D.T., Cech, T.R. and Davidovich, C. Targeting of Polycomb Repressive Complex 2 (PRC2) to RNA by Short Repeats of Consecutive Guanines. Mol. Cell, 65: 1056-1067 (2017).

  • 350.  Wang, X., Paucek, R. D., Gooding, A. R., Brown, Z. Z., Ge, E. J., Muir, T. W., & Cech, T. R. Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA. Nat Struct Mol Biol, 24: 1028-1038 (2017).

  • 349.  Stern, J. L., Paucek, R. D., Huang, F. W., Ghandi, M., Nwumeh, R., Costello, J. C., & Cech, T. R. Allele-Specific DNA Methylation and Its Interplay with Repressive Histone Marks at Promoter-Mutant TERT Genes. Cell Rep, 21: 3700-3707 (2017).

  • 348.  Long, Y., Wang, X., Youmans, D. T., & Cech, T. R. How do lncRNAs regulate transcription? Sci Adv, 3: eaao 2110 (2017).

  • 347. Long, Y., Bolanos, B., Gong, L., Liu, W., Goodrich, K. J., Yang, X., Chen, S., Gooding, A.R., Maegley, K.A., Gajiwala, K.S., Brooun, A., Cech, T.R., & Liu, X. Conserved RNA-binding specificity of polycomb repressive complex 2 is achieved by dispersed amino acid patches in EZH2. Elife, 6: (2017).

  • 346. Lim, C. J., Zaug, A. J., Kim, H. J., & Cech, T. R. Reconstitution of human shelterin complexes reveals unexpected stoichiometry and dual pathways to enhance telomerase processivity. Nat Commun, 8: 1075 (2017).

  • 345..Goldfarb, K. C., & Cech, T. R. (2017). Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing. Genes Dev, 31: 59-71.

2016

​

  • 344. Schmidt, J. C., Zaug, A. J. and Cech, T. R. Live cell imaging reveals dynamic interactions that drive telomerase recruitment to telomeres. Cell, 166,1188-1197 (2016).

  • 343. Lu, Z., Zhang, Q. C., Lee, B., Flynn, R. A., Smith, M. A., Robinson, J. T., ., ., ., ., ., ., Chang, H. Y. RNA Duplex Map in Living Cells Reveals Higher-Order Transcriptome Structure. Cell, 165: 1267-1279 (2016).

  • 342. Shukla, S., Schmidt, J.C., Goldfarb, K.C., Cech, T.R., Parker, R. Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects. (2016). , 4: 286-292 (2016).

2015

  • 341. Xi, L., Schmidt, J. C., Zaug, A. J., Ascarrunz, D. R., & Cech, T. R. A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biol, 16: 231 (2015).
  • 340. Stern, J. L., Theodorescu, D., Vogelstein, B., Papadopoulos, N., & Cech, T. R. Mutation of the TERT promoter, switch to active chromatin, and monoallelic TERT expression in multiple cancers. Genes Dev, 29: 2219-2224 (2015).
  • 339. Davidovich, C., & Cech, T. R. The recruitment of chromatin modifiers by long noncoding RNAs: lessons from PRC2. RNA, 21: 2007-2022 (2015).
  • 338. Xi, L., Schmidt, J. C., Zaug, A. J., Ascarrunz, D. R., & Cech, T. R. A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biol, 16: 231 (2015).
  • 337. Stern, J. L., Theodorescu, D., Vogelstein, B., Papadopoulos, N., & Cech, T. R. Mutation of the TERT promoter, switch to active chromatin, and monoallelic TERT expression in multiple cancers. Genes Dev, 29: 2219-2224 (2015).
  • 336.  Xi, L., & Cech, T. R. Protein-RNA interaction restricts telomerase from running through the stop sign. Nat Struct Mol Biol, 22: 835-836 (2015).
  • 335.  Wang, X., Schwartz, J.C., and Cech, T.R. Nucleic acid-binding specificity of human FUS protein. Nucleic Acids Res., (2015). doi: 10.1083/nar/gkv679 [epub ahead of print].
  • 334.  Tang, M., Li, Y., Zhang, Y., Chen, Y., Huang, W., Wang, D., Zaug, A.J., Liu, D., Zhao, Y., Cech, T.R. et al.  Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance.  Yournal of Cell Science, 128, 331-341, (2015).
  • 333.  Schwartz, J.C., Cech, T.R., and Parker, R.R. Biochemical properties and biological functions of FET proteins.  Annual Review of Biochemistry, 84, 355-379, (2015).
  • 332.  Schmidt, J.C., and Cech, T.R.  Human telomerase: biogenesis, trafficking, recruitment, and activation. Genes Dev., 29, 2095-1105, (2015).
  • 331.  Davidovich, C., Wang, X., Cifuentes-Rojas, C., Goodrich, K.J., Gooding, A.R., Lee, J.T. and Cech, T.R.  Toward a consensus on the binding specificity and promiscuity of PRC2 for RNA.  Molecular Cell, 57, 552-558, (2015).
  • 330.  Dalby, A.B., Hofr, C. and Cech, T.R.  Contributions of the TEL-patch amino acide cluster on TPP1 to telomeric DNA synthesis by human telomerase.  Journal of Mol. Biol. (2015). [Accepted for publication 01.09.2015].
  • 329.  Cech, T.R.  RNA World research - still evolving.  RNA, 21, 474-475. (2015).
  • 328.  Borah, S., Xi, L., Zaug, J.J., Powell, N.M., Dancik, G.M., Cohen, S.B., Costello, J.C., Theodorescu, D., Cech, T.R.  TERT mutations and telomerase reactiviation in Urotheilal Cancer.  Science, 347, 1006-1010, (2015).

2014

  • 327.  Schmidt, J.C., Dalby, A.B. and Cech, T.R. Identification of human TERT elements necessary for telomerase recruitment to telomeres.eLife, 3,(2014).
  • 326. Schwartz, J.C., Podell, E.R., Han, S.S., Berry, J.D., Eggan, K.C. and Cech, T.R. FUS is sequestered in nuclear aggregates in ALS patient fibroblasts. Molecular Biology of the Cell 25, 2571-2578, (2014).
  • 325. Davidovich, C., Goodrich, K.J., Gooding, A.R. and Cech, T.R. A dimeric state for PRC2. Nucleic Acids Research 42, 9236-9248, (2014).
  • 324. Xi, L. and Cech, T.R. Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT. Nucleic Acids Research 42, 8565-8577, (2014).
  • 323. Cech, T.R. and Steitz, J.A. The noncoding RNA revolution-trashing old rules to forge new ones. Cell 157, 77-94, (2014).
  • 322. Theodorescu, D., and Cech, T.R. Telomerase in bladder cancer: back to a better future? Eur Urol 65, 370-371. (2014).
  • 321. McKay, D.B., Xi, L., Barthel, K.K., Cech, T.R. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. Journal of Molecular Biology. 426 1766-1785 (2014).

2013

  • 320. Schwartz, J.C., Wang, X., Podell, E. R., Cech, T.R. RNA seeds higher-order assembly of FUS protein. Cell Reports 5, EPub Nov 21 (2013).
  • 319. Nakashima, M., Nandakumar, J., Sullivan, K. D., Espinosa, J. M. & Cech, T. R. Inhibition of Telomerase Recruitment and Cancer Cell Death. Journal of Biological Chemistry EPub Oct 4 (2013).
  • 318. Goldfarb, K.C., and Cech, T.R. 3[prime] terminal diversity of MRP RNA and other  human noncoding RNAs revealed by deep sequencing.BMC Molecular Biology 14, 23 (2013).
  • 317. Davidovich, C., Zheng, L., Goodrich, K.J., and Cech, T.R. Promiscuous RNA binding by Polycomb repressive complex 2. Nature Structural & Molecular Biology 20, 1250-1257 (2013).
  • 316. Zaug, A.J., Crary, S.M., Fioravanti, M.J., Campbell, K., Cech, T.R. Many disease- associated variants of hTERT retain high telomerase enzymatic activity. Nucleic Acids Res. 19, 8969-8978
  • 315. Dalby, A.B., Goodrich, K.J., Pfingsten, J.S., Cech, T.R., RNA recognition by the DNA end-binding Ku   heterodimer.  6: 841-851 (2013).
  • 314. Nandakumar, J., Cech, T.R. Finding the end: recruitment of telomerase to telomeres. Nature Reviews Molecular Cell Biology, 14: 69-82 (2013).

2012

  • 313. Schwartz, J.C., Ebmeier, C.C., Podell, E.R., Heimiller, J., Taatjes, D.J., Cech, T.R. FUS binds the CTD of RNA polymerase II and regulates its phosphorylation at Ser2. Genes Dev. 26: 2690-95 (2012).
  • 312. Nandakumar, J., Bell, C.F., Weidenfeld, I., Zaug, A.J., Leinwand, L.A., Cech, T.R. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity. Nature, 492: 285-289 (2012).
  • 311. Goldfarb, K.C., Borah, S., Cech, T.R. RNase P branches out from RNP to protein: organelle-triggered diversification? Genes Dev. 10, 1005-1009 (2012).
  • 310. Pfingsten, J.S., Goodrich, K.J., Taabazuing, C., Ouenzar, F., Chartrand, P., Cech, T.R. Mutually Exclusive Binding of Telomerase RNA and DNA by Ku Alters telomerase recruitment Model. Cell, 148, 922-932 (2012).
  • 309. Nandakumar, J., Cech, T.R. DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe. Nucleic Acids Res. 40, 235-244 (2012).

2011

  • 308. Berman A.J., Akiyama B.M., Stone M.D., Cech T.R. The RNA accordion model for template positioning by telomerase RNA during telomeric DNA synthesis. Nature Structural and Molecular Biology 18, 1371-1375 (2011). Batista L.F., Pech M.F., Zhong F.L., Nguyen H.N., Xie K.T., Zaug A.J., Crary S.M., Choi J., Sebastiano V., Cherry A., Giri N., Wernig M., Alter B.P., Cech T.R., Savage S.A., Reijo Pera R.A., Artandi S.E. Telomere shortening and loss of self-renewal in dyskeratosis congenita induced pluripotent stem cells. Nature 474, 399-402 (2011).
  • 307. Taylor D.J., Podell E.R., Taatjes D.J., Cech T.R. Multiple POT1-TPP1 proteins coat and compact long telomeric single-stranded DNA. J. Mol. Biol. 410, 10-17 (2011). Cech T.R. The RNA Worlds in Context. CSH Perspect. Biol. 3, a006742 (2011).
  • 306. Zappulla D.C., Goodrich K.J., Arthur J.R., Gurski L.A., Denham E.M., Stellwagen A.E., Cech T.R. Ku can contribute to telomere lengthening in yeast at multiple positions in the telomerase RNP. RNA 14, 298-311 (2011).

2010

  • 305. Berman A.J., Gooding A.R., and Cech T.R. Tetrahymena telomerase protein p65 induces conformational changes throughout telomerase RNA (TER) and rescues telomerase reverse transcriptase and TER assembly mutants. Molecular and Cellular Biology 30, 4965-4976 (2010).
  • 304. Zaug A.J., Podell E.R., Nandakumar J., and Cech T.R. Functional interaction between telomere protein TPP1 and telomerase. Genes & Development 24, 613-622 (2010).
  • 303. Qiao F., Goodrich K.J., and Cech T.R. Engineering cis-telomerase RNAs that add telomeric repeats to themselves. Proc. Natl Acad. Sci. U.S.A 107, 4914-4918 (2010).
  • 302. Latrick C.M., Cech T.R. POT1-TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation. EMBO J. 29, 924-933 (2010).
  • 301. Nandakumar J., Podell E.R., Cech T.R. How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA. Proc. Natl. Acad. Sci. USA 107, 651-656 (2010).

2009

  • Andrews N., Burris J.E., Cech T.R., Coller B.S., Crowley W.F. Jr, Gallin E.K., Kelner K.L., Kirch D.G., Leshner A.I., Morris C.D., Nguyen F.T., Oates J., Sung N.S.. Translational careers.Science 324, 855 (2009).
  • 298. Vicens Q. and Cech T.R. A natural ribozyme with 3',5' RNA ligase activity. Nature Chemical Biology 5, 97-99 (2009).
  • 299. Cech T.R. Evolution of biological catalysis: ribozyme to RNP enzyme. Cold Spring Harbor Symposia on Quantitative Biology 74, 11-16 (2009).
  • 300. Cech T.R. Crawling out of the RNA world. Cell 136, 599-602 (2009).

2008

  • 293. Qiao F. and Cech T.R. Triple-helix structure in telomerase RNA contributes to catalysis.Nature Structural and Molecular Biology 15, 634-640 (2008).
  • 294. Mozdy A.D., Podell E.R., and Cech T.R. Multiple yeast genes, including Paf1 complex genes, affect telomere length via telomerase RNA abundance. Molecular and Cellular Biology 28, 4152-4161 (2008).
  • 295. Zaug A.J., Podell E.R., and Cech T.R. Mutation in TERT separates processivity from anchor-site function. Nature Structural and Molecular Biology 15, 870-872 (2008).
  • 296. Vicens Q., Paukstelis P.J., Westhof E., Lambowitz A.M., and Cech T.R. Toward predicting self-splicing and protein-facilitated splicing of group I introns. RNA 14, 2013-2029 (2008).
  • 297. Zappulla D.C., Roberts J.N., Goodrich K.J., and Cech T.R., and Wuttke D.S. Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids Research37, 354-367 (2008).

2007

  • 289. Zappulla, D. and Cech, T.R. RNA as a Flexible Scaffold for Proteins: Yeast Telomerase and Beyond. Cold Spring Harbor Symposia on Quantitative Biology, LXXI (2007).
  • 290. Wang, F., Podell, E.R., Zaug, A.J., Yang, Y., Baciu, P., Cech, T.R. and Lei, M. The POT1-TPP1 Telomere Complex is a Telomerase Processivity Factor. Nature, 445, 506-510 (2007).
  • 291. Wang F, Podell E.R., Zaug A.J., Yang Y., Baciu P., and Cech T.R., Lei M. The POT1-TPP1 telomere complex is a telomerase processivity factor. Nature 445, 506-510 (2007). [PDB ID:2i46]
  • 292. Vicens Q., Gooding A. R., Laederach A., and Cech T.R. Local RNA structural changes induced by crystallization are revealed by SHAPE. RNA. 2007 Apr;13(4): 536-48.

2006

  • 284. Cech, T.R., Moras, D., Nagai, K. and Williamson, J.R. “The RNP World,” In The RNA World, Third Edition (Gesteland, R., Cech, T.R. and Atkins, J., ed.; Cold Spring Harbor Laboratory Press), Chapter 11, 309-326 (2006).
  • 285. Jacobs S.A., Podell E.R. and Cech T.R. Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase. Nat Struct Mol Biol. 2006 Feb 5. [PDB ID: 2b2a]
  • 286. Vicens Q. and Cech T.R. Atomic level architecture of group I introns revealed. Trends Biochem Sci. 2006 Jan;31(1):41-51.
  • 287. Guo F, Gooding A.R. and Cech T.R. Comparison of crystal structure interactions and thermodynamics for stabilizing mutations in the Tetrahymena ribozyme. RNA. 2006 Mar;12(3): 387-95.
  • 288. Mozdy A.D. and Cech T.R. Low abundance of telomerase in yeast: Implications for telomerase haploinsufficiency. RNA. 2006 Sept;12(9): 1721-37.

2005

  • 278. Mandell, J.G., Goodrich K.J., Bähler J. and Cech T.R. Expression of a RecQ helicase homolog affects progression through crisis in fission yeast lacking telomerase. J Biol Chem. 280, 5249-5257 (2005).
  • 279. Lei M, Zaug AJ, Podell ER, and Cech T. R. Switching human telomerase on and off with hPOT1 protein in vitro. J Biol Chem. 2005 May 27;280(21):20449-56.
  • 280. Zaug AJ, Podell ER and Cech TR. Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10864-9.
  • 281. Jacobs SA, Podell ER, Wuttke DS and Cech T.R. Soluble domains of telomerase reverse transcriptase identified by high-throughput screening. Protein Sci. 2005 Aug;14(8):2051-8.
  • 282. Opresko PL, Mason PA, Podell ER, Lei M, Hickson ID, Cech T. R and Bohr VA. POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates. J Biol Chem.2005 Sep 16;280(37):32069-80.
  • 283. Zappulla DC, Goodrich K and Cech T. R. A miniature yeast telomerase RNA functions in vivo and reconstitutes activity in vitro. Nat Struct Mol Biol. 2005 Dec;12(12):1072-7.

2004

  • 271. Cech, T. R. Beginning to Understand the End of the Chromosome (Review). Cell 116, 273-279 (2004).
  • 272. Cech, T. R. RNA Finds a Simpler Way. Nature 428, 263-264 (2004).
  • 273. Zappulla, D. C. and Cech, T. R. Yeast Telomerase RNA: A Flexible Scaffold for Protein Subunits. Proc. Natl. Acad. Sci. USA 101, 10024-10029 (2004).
  • 274. Aigner, S. and Cech, T. R. The Euplotes Telomerase Subunit p43 Stimulates Enzymatic Activity and Processivity in vitro. RNA 10, 1108-1118 2004
  • 275. Guo, F., Gooding, A. R. and Cech, T. R. Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site. Molec. Cell 16, 351-362 (2004). [PDB ID:1x8w]
  • 276. Lei, M., Podell, E. R. and Cech, T. R. Structure of Human POT1 Bound to Telomeric Single-stranded DNA provides a model for chromosome end-protection. Nature Struc, Mol. Biol. 11, 1223-1229 (2004). [PDB ID: 1xjv]
  • 277. Mandell, J. G., Bähler, Volpe, T. A., Martienssen, R. A. and Cech, T. R. The Euplotes Telomerase Subunity p43 Stimulates Enzymatic Activity and Processivity in vitro. Genome Biol. 6 RI.1-RI.15 (2004).

2003

  • 262. Cech, T.R. Ribozymes, The First 20 Years. Biochemical Society Transactions 30, 1162-1166 (2002).
  • 263. Friedman, K. L., Heit, J. J. , Long, D. L. and Cech, T. R. N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex. Molec. Biol. Cell 14, 1-13 (2003).
  • 264. Förstemann, K., Zaug, A. J., Cech, T. R. and Lingner, J. Yeast Telomerase is Specialized for C/A-rich RNA Templates. Nucleic Acids Res. 31, 1646-1655 (2003).
  • 265. Aigner, S., Postberg, J., Lipps, H. J. and Cech, T. R. The Euplotes La Motif Protein p43 Has Properties of a Telomerase-Specific Subunit. Biochemistry 42, 5736-5747 (2003).
  • 266. Colgin, L. M., Baran, K., Baumann, P., Cech, T. R. and Reddel, R. R. Human POT1 Facilitates Telomere Elongation by Telomerase. Current Biology 13, 942-946 (2003).
  • 267. Classen, S., Lyons, D., Cech, T. R. and Schultz, S. C. Sequence Specific and 3'-end Selective Single Strand DNA Binding by the Oxytricha nova Telomere End Binding Protein a Subunit. Biochemistry 42, 9269-9277 (2003).
  • 268. Seto, A. G., Tzfati, Y., Zaug, A. J., Blackburn, E. H. and Cech, T. R. A Template-proximal RNA Paired Element Contributes to S. cerevisiae Telomerase Activity. RNA 9, 1323-1332 (2003).
  • 269. Lei, M., Podell, E. R., Baumann, P. and Cech, T. R. DNA Self-recognition in the Structure of Pot1 Bound to Telomeric Single-stranded DNA. Nature 426, 198-203 (2003).
  • 270. Bryan, T. M., Goodrich, K. J. and Cech, T. R. Tetrahymena telomerase is Active as a Monomer. Molec. Biol. Cell 14, 4794-4804 (2003). [PDB ID: 1qzh, 1qzg]

2002

  • 254. Jarstfer, M.B. and Cech, T.R. Effects of Nucleotide Analogues on Euplotes aediculatusTelomerase Processivity: Evidence for Product-Assisted Translocation. Biochemistry 41, 151-161
  • 255. Livengood, A.J., Zaug, A.J. and Cech, T.R. Essential Regions of Saccharomyces cerevisiaeTelomerase RNA: Separate Elements for Est1p and Est2p Interaction. Molecular Cell Biology 22, 2366-2374
  • 256. Guo, F. and Cech, T. R. In vivo Selection of Better Self-Splicing Introns in E. coli: the Role of the P1 Extension Helix of the Tetrahymena Intron. RNA 8, 647-658
  • 257. Doudna, J. A. and Cech, T. R. The Chemical Repertoire of Natural Ribozymes. Nature 418, 222-228.
  • 258. Baumann, P., Podell, E. and Cech, T. R. Human Pot1 (Protection of Telomeres) Protein: Cytolocalization Gene Structure, and Alternative Splicing. Molec. Cell. Biol. 22, 8079-8087
  • 259. Guo, F. and Cech, T. R. Evolution of Tetrahymena Ribozyme Mutants with Increased Structural Stability. Nature Structural Biology 9, 855-961
  • 260. Seto, A. G., Livengood, A. J., Tzfati, Y., Blackburn, E. H. and Cech, T. R. A Bulged Stem Tethers Est1p to Telomerase RNA in Budding Yeast. Genes Dev. 16, 2800-2812
  • 261. Lei, M., Baumann, P. and Cech, T. R. Cooperative Binding of Single-stranded TelomericDNA by Pot1 Protein of Schizosaccharomyces pombe. Biochemistry 41, 14560-14568

2001

  • 249. Silverman, S.K. and Cech, T.R. An Early Transition State for Folding of the P4-P6 RNA Domain. RNA 7, 161-166
  • 250. Juneau, K., Podell E., Harrington, D.J. and Cech, T.R. Strucutal Basis of the Enhanced Stability of a Mutant Ribozyme Domain and a Detailed View of RNA-Solvent InteractionsStructure 9, 221-231. [PDB ID: 1hr2]
  • 251. Cech, T.R. Overturning the Dogma: Catalytic RNA. In The Origins of Creativity, (Pfenninger, K.H. and Shubik, V.R. eds.) Oxford University Press , pp. 5-17
  • 252. Baumann, P. and Cech, T.R. Pot1, the Putative Telomere End-binding Protein in Fission Yeast and Humans. Science 292, 1171-1175
  • 253. Sperger, J.M. and Cech, A Stem-loop of Tetrahymena Telomerase RNA Distant from the Template Potentiates RNA Folding and Telomerase Activity. Biochemistry 40, 7005-7016

2000

  • 240. Cech, T.R. Life at the End of the Chromosome: Telomeres and Telomerase. Angewandt Chemie (International Edition) 39, 34-43.
  • 241. Cohen, S. B. and Cech, T. R. Engineering Disulfide Cross-links in RNA Using Thiol-Disulfide Interchange Chemistry. In Current Protocols in Nucleic Acid Chemistry (S. L. Beaucage, D. E. Bergstrom, G. D. Glick and R. A. Jones, eds.) John Wiley & Sons, Inc. New York, Unit 5.1, pp. 1-10.
  • 242. Haering, C.H., Nakamura, T.M., Baumann, P., and Cech, T.R. Analysis of telomerase catalytic subunit mutants in vivo and in vitro in Schizosaccharomyces pombe. Proc Natl Acad Sci U S A 97(12), 6367-72.
  • 243. Bryan, T. M., Goodrich, K. J. and Cech, T. R. A Mutant of Tetrahymena Telomerase Reverse Transcriptase with Increased Processivity.J. Biol. Chem. 275, 24199-24207.
  • 244. Cech, T.R. Structural biology. The ribosome is a ribozyme. Science 289(5481), 878-9.
  • 245. Bryan, T.M., Goodrich, K.J., and Cech T.R. Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase. Mol Cell 6(2), 493-9.
  • 246. Silverman, S.K., Deras, M.L., Woodson, S.A., Scaringe, S.A., and Cech T.R. Multiple folding pathways for the P4-P6 RNA domain.Biochemistry 39(40), 12465-75.
  • 247. Baumann, P., Cech T.R. Protection of telomeres by the Ku protein in fission yeast. Mol Biol Cell 11(10), 3265-75.
  • 248. Aigner, S., Lingner, J., Goodrich, K., Grosshans, C.A., Shevchenko, A., Mann, M., and Cech, T.R. Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting. EMBO19(22),6230-9.

1999

  • 231. Cech, T. R. and Golden, B. L. Building a Catalytic Active Site Using Only RNA. In The RNA World, Second Edition (R. Gesteland and J. Atkins, T. R. Cech, eds.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 321-349.
  • 232. Bryan, T. M. and Cech, T. R. Telomerase and the maintenance of chromosome ends. Curr. Opin. Cell Biol. 11, 318-324.
  • 233. Silverman, S. K. and Cech, T. R. Energetics and Cooperativity of Tertiary Hydrogen Bonds in RNA Structure. Biochemistry38, 8691-8702.
  • 234. Juneau, K. and Cech, T. R. In vitro selection of RNAs with increased tertiary structure stability. RNA5,1119-1129.
  • 235. Seto, A. G., Zaug, A. J., Sobel, S. G., Wolin, S. L. and Cech, T. R. Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle. Nature401, 177-180.
  • 236. Silverman, S.K. and Cech, T.R. RNA Tertiary Folding Monitored by Flourescence of Covalently Attached Pyrene. Biochemistry38, 14224-14237.
  • 237. Friedman, K.L. and Cech, T.R. Essential functions of N-terminal Domains in the Yeast Telomerase Catalytic Subunit Revealed by Selection for Viable Mutants. Genes Dev. 13, 2863-2874.
  • 238. Hagen, M. and Cech, T.R. Self-splicing of the Tetrahymena intron from mRNA in Mammalian Cells. EMBO J. 18, 6491-6500.
  • 239. Silverman, S.K., Zheng, M., Wu, M., Tinoco, Jr., I. and Cech, T.R. Quantifiying the Energetic Interplay of RNA Tertiary and Secondary Structure Interactions. RNA5, 1665-1674.

1998

  • 219. Nakamura, T. M. and Cech, T. R. Reversing Time: Origin of Telomerase. (Minireview)Cell92, 587-590.
  • 220. Hammond, P. W. and Cech, T. R. Euplotes Telomerase: Evidence for Limited Base-Pairing During Primer Elongation and dGTP as an Effector of Translocation. Biochemistry37, 5162-5172.
  • 221. Froelich-Ammon, S. J., Dickinson, B. A., Bevilacqua, J. M., Schultz, S. C. and Cech, T. R. Modulation of Telomerase Activity by Telomere DNA-Binding Proteins in Oxytricha.Genes Dev.12, 1504-1514.
  • 222. Bevilacqua, P. C., George, C. X., Samuel, C. E. and Cech, T. R. Binding of the Protein Kinase PKR to RNAs with Secondary Structure Defects: Role of the Tandem A-G Mismatch and Noncontiguous Helixes. Biochemistry37, 6303-6316.
  • 223. Lingner, J. and Cech, T. R. Telomerase and Chromosome End Maintenance. Curr. Opin. Genet. Dev.8, 226-232.
  • 224. Bryan, T. M., Sperger, J. M., Chapman, K. B. and Cech, T. R. Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax. Proc. Natl. Acad. Sci. USA 95, 8479-8484.
  • 225. Szewczak, A. A., Podell, E. R., Bevilacqua, P. C. and Cech, T. R. Thermodynamic stability of the P4-P6 domain RNA tertiary structure measured by temperature gradient gel electrophoresis.Biochemistry37, 11162-11170.
  • 226. Zhang, B. and Cech, T. R. Peptidyl-transferase Ribozymes: trans Reactions, Structural Characterization and Ribosomal RNA-like Features. Chemistry & Biology 5, 539-553.
  • 227. Cohen, S. B. and Cech, T. R. A quantitative study of the flexibility contributed to RNA structures by nicks and single-stranded gaps. RNA4, 1179-1185.
  • 228. Golden, B. L., Gooding, A. R., Podell, E. and Cech, T. R. A Preorganized Active Site in the Crystal Structure of the Tetrahymena Ribozyme. Science 282, 259-264. [PDB ID: 1grz]
  • 229. Nakamura, T. M., Cooper, J. P. and Cech, T. R. Two Modes of Survival of Fission Yeast without Telomerase. Science282, 493-496.
  • 230. Jabri, E. and Cech, T. R. In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity. RNA4, 1481-1492.

1997

  • 201. Hammond, P. W., Lively, T. N. and Cech, T. R. The Anchor Site of Telomerase fromEuplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers. Molec. Cell. Biol. 17, 296-308.
  • 202. Tanner, M. A. and Cech, T. R. Joining the Two Domains of a Group I Ribozyme to Form the Catalytic Core. Science275, 847-849.
  • 203. Cooper, J. P., Nimmo, E. R., Alishire, R. C. and Cech, T. R. Regulation of Telomere Length and Function by a Myb-Domain Protein in Fission Yeast. Nature385, 744-747.
  • 204. Lingner, J., Hughes, T. R., Shevchenko, A., Mann, M., Lundblad, V., Cech, T. R. Reverse Transcriptase Motifs in the Catalytic Subunit of Telomerase. Science276, 561-567.
  • 205. McConnell, T. S., Herschlag, D. and Cech, T. R. Effects of Divalent Metal Ions on Individual Steps of the Tetrahymena Ribozyme Reaction. Biochemistry36, 8293-8303.
  • 206. Golden, B. L., Gooding, A. R., Podell, E. and Cech, T. R. Crystals by Design: A Strategy for Crystallization of a Ribozyme Derived from the Tetrahymena group I intron. J. Mol Biol 270, 711-723.
  • 207. Cohen, S. B. and Cech, T. R. Dynamics of Thermal Motions within a Large Catalytic RNA Investigated by Cross-linking with Thiol-Disulfide Interchange. J. Am. Chem. Soc.119, 6259-6268.
  • 208. Szewczak, A. A. and Cech, T. R. An Internal Loop Acts as a Hinge to Facilitate Ribozyme Folding and Catalysis. RNA3, 838-849.
  • 209. Nakamura, T. M., Morin, G. B., Chapman, K. B., Weinrich, S. L., Andrews, W. H., Lingner, J., Harley, C. B. and Cech. T. R. Telomerase Catalytic Subunit Homologs from Fission Yeast and Human. Science277, 955-959.
  • 210. Weinstein, L. B., Jones, B. C. N. M., Cosstick, R. and Cech, T. R. A Second Catalytic Metal ion in a Group I Ribozyme. Nature388, 805-807.
  • 211. Tanner, M. A., Anderson, E. M., Gutell, R. R. and Cech, T. R. Mutagenesis and Comparative Sequence Analysis of a Base Triple Joining the Two Domains of Group I Ribozymes. RNA3, 1037-1051.
  • 212. Hammond, P. W. and Cech, T. R. dGTP-Dependent Processivity and Possible Template Switching of Euplotes Telomerase. Nucleic Acids Res. 25, 3698-3704.
  • 213. Lingner, J., Cech, T. R., Hughes, T. R. and Lundblad, V. Three Ever Shorter Telomere (EST) Genes Are Dispensable for in vitro Yeast Telomerase Activity. Proc. Natl. Acad. Sci. USA 94, 11190-11195.
  • 214. Chapon, C., Cech, T. R. and Zaug, A. J. Polyadenylation of Telomerase RNA in Budding Yeast. RNA3, 1337-1351.
  • 215. Zhang, B. and Cech, T. R. Peptide Bond Formation by in vitro Selected Ribozymes.Nature390, 96-100.
  • 216. Cech, T. R., Nakamura, T. M. and Lingner, J. Telomerase Is a True Reverse Transcriptase.Biochemistry (Moscow) 62, 1202-1205.
  • 217. Jabri, E., Aigner, S. and Cech, T. R. Kinetic and Secondary Structure Analysis of Naegleria andersoni GIR1: a Group I Intron Whose Putative Biological Function is Site-specific Hydrolysis.Biochemistry36. 16345-16354.
  • 218. Cech, T. R. and Lingner, J. Telomerase and the Chromosome End-replication Problem.Proceedings of the CIBA Foundation Symposium No. 211, (John Wiley & Sons) pp. 20-34.

1996

  • 183. Zaug, A. J., Lingner, J. and Cech, T. R. Method for Determining RNA 3' Ends and Application to Human Telomerase RNA. Nucleic Acids Res. 24, 532-533.
  • 184. Tanner, M. A. and Cech, T. R. Activity and Thermostability of the Small Self-splicing Group I Intron in the Pre-tRNAIle of the Purple Bacterium Azoarcus. RNA2, 74-83.
  • 185. Weeks, K. M. and Cech, T. R. Assembly of a Ribonucleoprotein Catalyst by Tertiary Structure Capture. Science271, 345-348.
  • 186. Strobel, S. A. and Cech, T. R. The Exocyclic Amine of the Conserved G.U Pair at the Cleavage Site of the Tetrahymena Ribozyme Contributes to 5'-Splice Site Selection and Transition State Stabilization. Biochemistry35, 1201-1211.
  • 187. Golden, B. L. and Cech, T. R. Conformational Switches Involved in Orchestrating the Successive Steps of Group I RNA Splicing. Biochemistry35, 3754-3763.
  • 188. Rusconi, C. P. and Cech, T. R. Mitochondrial Import of Only One of Three Nuclear-Encoded Glutamine tRNAs in Tetrahymena thermophila. EMBO J. 15, 3286-3295.
  • 189. Cech, T. R. and Herschlag, D. Group I Ribozymes: Substrate Recognition, Catalytic Strategies and Comparative Mechanistic Analysis. In Nucleic Acids and Molecular Biology, vol. 10 (F. Eckstein and D. M. J. Lilley, eds) Berlin, Springer-Verlag, pp. 1-17.
  • 190. Cech, T. R. and Szewczak, A. A. Selecting Apt RNAs for NMR. (Perspective), RNA2, 625-627.
  • 191. Downs, W. D. and Cech, T. R. Kinetic Pathway for Folding of the Tetrahymena Ribozyme Revealed by Three Ultraviolet-Inducible Cross-Links. RNA2, 718-732.
  • 192. Bevilacqua, P. C. and Cech, T. R. Minor Groove Recognition of Double-Stranded RNA by the Double-Stranded RNA-Binding Domain from the RNA-Activated Protein Kinase PKR.Biochemistry35, 9983-9994.
  • 193. Campbell, T. B. and Cech, T. R. Mutations in the Tetrahymena Ribozyme Internal Guide Sequence: Effects on Docking of the P1 Helix into the Catalytic Core and Correlation with Catalytic Activity. Biochemistry35, 11493-11502.
  • 194. Cate, J. H., Gooding, A. R., Podell, E., Zhou, K., Golden, B. L., Kundrot, C. E., Cech, T. R. and Doudna, J. A. Crystal Structure of a Group I Ribozyme Domain Reveals Principles of Higher Order RNA Folding. Science273, 1678-1685. [PDB ID: 1gid]
  • 195. Cate, J. H., Gooding, A. R., Podell, E., Zhou, K., Golden, B. L., Szewczak, A. A., Kundrot, C. E., Cech, T. R. and Doudna, J. A. Adenosine Platforms Mediate the Formation of RNA Tertiary Structure. Science273, 1696-1699.
  • 196. Lingner, J. and Cech, T. R. Purification of Telomerase from Euplotes aediculatus: Requirement of a Primer 3' Overhang. Proc. Natl. Acad. Sci. USA 93, 10712-10717.
  • 197. Kuo, L. Y. and Cech, T. R. Conserved Thermochemistry of Guanosine Nucleophile Binding for Structurally Distinct Group I Ribozymes. Nucleic Acids Res. 24, 3722-3727.
  • 198. Weinstein, L. B., Eamshaw, D. J., Cosstick, R., and Cech, T. R. Synthesis and Characterization of an RNA Dinucleotide Containing a 3'-S-Phosphorothiolate Linkage. J. Am. Chem. Soc. 118, 10341-10350.
  • 199. Rusconi, C. P. and Cech, T. R. The Anticodon is the Signal Sequence for Mitochondrial Import of Glutamine tRNA in Tetrahymena. Genes Dev. 10, 2870-2880.
  • 200. Golden, B. L., Gooding, A. R., Podell, E. and Cech, T. R. X-ray Crystallography of Large RNAs: Heavy Atom Derivatives by RNA Engineering. RNA2, 1295-1305.

1995

  • 167. Strobel, S. A. and Cech, T. R. Minor Groove Recognition of the Conserved G.U Pair at theTetrahymena Ribozyme Reaction Site. Science 267, 675-679.
  • 168. Doudna, J. A., Cech, T. R. and Sullenger, B. A. Selection of an RNA Molecule that Mimics a Major Autoantigenic Epitope of Human Insulin Receptor. Proc. Natl. Acad. Sci. USA 92, 2355-2359.
  • 169. McConnell, T. S. and Cech, T. R. A Positive Entropy Change for Guanosine Binding and for the Chemical Step in the Tetrahymena Ribozyme Reaction. Biochemistry 34, 4056-4067.
  • 170. Doudna, J. A. and Cech, T. R. Self-assembly of a Group I Intron Active Site from its Component Tertiary Structural Domains. RNA 1, 36-45.
  • 171. Cech, T. R. Group I Introns: New Molecular Mechanisms for mRNA Repair. Biotechnology13, 323-326.
  • 172. Cech, T. R. RNA:Getting the Message. In Triumph of Discovery, a Chronicle of Great Adventures in Science, Scientific American 150th Anniversary Book. (Henry Holt and Company, New York) pp. 192-195.
  • 173. Weeks, K. W. and Cech, T. R. Efficient Protein-Facilitated Splicing of the Yeast Mitochondrial bI5 Intron. Biochemistry 34, 7728-7738.
  • 174. Hicke, B. J., Rempel, R., Maller, J., Swank, R., Hamaguchi, J., Bradbury, E. M., Prescott, D. M. and Cech, T. R. Phosphorylation of the Oxytricha Telomere Protein: Possible Cell Cycle Regulation. Nucleic Acids Res. 23, 1887-1893.
  • 175. Fang, G. and Cech, T. R. Telomerase RNA in the Replication Band and Spherical Subnuclear Organelles in Hypotrichous Ciliates. J. Cell Biol. 130, 243-253.
  • 176. Zaug, A. J. and Cech, T. R. Analysis of the Structure of Tetrahymena Nuclear RNAs in vivo: Telomerase RNA, the Self-Splicing rRNA Intron, and U2 snRNA. RNA 1, 363-374.
  • 177. Weeks, K. M. and Cech, T. R. Protein-Facilitation of Group I Intron Splicing by Assembly of RNA Domains. Cell 82, 221-230.
  • 178. Tanner, M. A. and Cech, T. R. An Important RNA Tertiary Interaction of Group I and Group II Introns also Occurs in Gram-positive RNase P RNAs. (Letter) RNA 1, 349-350.
  • 179. Fang, G. and Cech, T. R. Telomere Proteins. In Telomeres, (E. Blackburn and C. Greider, eds.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 69-105.
  • 180. Lingner, J., Cooper, J. P. and Cech, T. R. Telomerase and DNA End Replication: Not a Lagging Strand Problem? Science 269, 1533-1534.
  • 181. Campbell, T. B. and Cech, T. R. Identification of Ribozymes within a Ribozyme Library that Efficiently Cleave a Long Substrate RNA. RNA 1, 598-609.
  • 182. Nakamura, T. M., Wang, Y.-H., Zaug, A. J., Griffith, J. D. and Cech, T. R. Relative Orientation of RNA Helices in a Group I Ribozyme Determined by Helix Extension Electron Microscopy. EMBO J. 14, 4849-4859.

1994

  • 149. Strobel, S. A. and Cech, T. R. Translocation of an RNA Duplex on a Ribozyme. Nature Structural Biology 1, 13-17.
  • 150. Murphy, F. L. and Cech, T. R. GAAA Tetraloop and Conserved Bulge Stabilize Tertiary Structure of a Group I Intron Domain. J. Molec. Biol. 236, 49-63.
  • 151. Wang, Y.-H., Murphy, F. L., Cech, T. R. and Griffith, J. D. Visualization of a Tertiary Structural Domain of the Tetrahymena Group I Intron by Electron Microscopy. J. Mol. Biol. 236, 64-71.
  • 152. Hicke, B. J., Willis, M. C., Koch, T. H. and Cech, T. R. Telomeric Protein-DNA Point Contacts Identified by Photo-Cross-Linking using 5-Bromodeoxyuridine. Biochemistry 33, 3364-3373. [Misprinted figure was corrected in Biochemistry 33, 7744.]
  • 153. Cech, T. R., Bevilacqua, P. C., Doudna, J. A., McConnell, T. S., Strobel, S. A. and Weinstein, L. B. Mechanism and Structure of a Catalytic RNA Molecule. Robert A. Welch Foundation Conferences on Chemical Research, Vol. XXXVII, pp. 91-110.
  • 154. Cech, T. R., Damberger, S. and Gutell, R. R. Representation of the Secondary and Tertiary Structure of Group I Introns. Nature Struc. Biol. 1, 273-280.
  • 155. Downs, W. D. and Cech, T. R. A Tertiary Interaction in the Tetrahymena Intron Contributes to the Accuracy of Self-splicing. Genes Dev. 8, 1198-1211.
  • 156. Wang, J.-F. and Cech, T. R. Metal Ion Dependence of Active-site Structure of theTetrahymena Ribozyme Revealed by Site-specific Photocrosslinking. J. Am. Chem. Soc. 116, 4178-4182.
  • 157. Laggerbauer, B., Murphy, F. L. and Cech, T. R. Two Major Tertiary Folding Transitions of the Tetrahymena Catalytic RNA. EMBO J. 13, 2669-2676.
  • 158. Lingner, J., Hendrick, L. L. and Cech, T. R. Telomerase RNAs of Different Ciliates Have a Common Secondary Structure and a Permuted Template. Genes Dev. 8, 1984-1998.
  • 159. Murphy, F. L., Wang, Y.-H., Griffith, J. D. and Cech, T. R. Coaxially Stacked RNA Helices in the Catalytic Center of the Tetrahymena Ribozyme. Science 265, 1709-1712.
  • 160. Cech, T. R. The Origin of Life and the Value of Life. In Biology, Ethics and the Origins of Life, (H. Rolston, III, Ed., Jones & Bartlett, Boston), pp. 15-37.
  • 161. Cech, T. R. Chromosome End Games (Perspective). Science 266, 387-388.
  • 162. Sullenger, B. A. and Cech, T. R. Ribozyme-Mediated Repair of Defective mRNA by Targeted Trans-Splicing. Nature 371, 619-622.
  • 163. Cech, T. R. and Uhlenbeck, O. C. Hammerhead Nailed Down (News & Views). Nature372, 39-40.
  • 164. Strobel, S. A., Cech, T. R., Usman, N. and Beigelman, L. The 2,6-Diaminopurine Riboside.5-Methyl-Isocytidine Wobble Base Pair: An Isoenergetic Substitution for the Study of G.U Pairs in RNA. Biochemistry 33, 13824-13835.
  • 165. Pyle, A. M., Moran, S., Strobel, S. A., Chapman, T., Turner, D. H. and Cech, T. R. Replacement of the Conserved G.U with a G-C Pair at the Cleavage Site of the TetrahymenaRibozyme Decreases Binding, Reactivity, and Fidelity. Biochemistry 33, 13856-13863.
  • 166. Zaug, A. J., Dávila-Aponte, J. A. and Cech, T. R. Catalysis of RNA Cleavage by a Ribozyme Derived from the Group I Intron of Anabaena Pre-tRNALeu. Biochemistry 33, 14935-14947.

1993

  • 130. Piccirilli, J. A., Vyle, J. S., Caruthers, M. H. and Cech, T. R. Metal Ion Catalysis by theTetrahymena Ribozyme. Nature 361, 85-88.
  • 131. Wang, J. F., Downs, W. D. and Cech, T. R. Movement of the Guide Sequence During RNA Catalysis by a Group I Ribozyme. Science 260, 504-508.
  • 132. Fang, G., Gray, J. and Cech, T. R. Oxytricha Telomere-binding Protein: Separable DNA-binding and Dimerization Domains of the alpha Subunit. Genes & Dev. 7, 870-882.
  • 133. Murphy, F. L. and Cech, T. R. An Independently Folding Domain of RNA Tertiary Structure Within the Tetrahymena Ribozyme. Biochemistry 32, 5291-5300.
  • 134. Cech, T. R. Catalytic RNA: Structure and Mechanism. Biochem. Soc. Trans. 21, 229-234.
  • 135. Cech, T. R. Structure and Mechanism of the Large Catalytic RNAs: Group I and Group II Introns and Ribonuclease P. In The RNA World (R. Gesteland and J. Atkins, eds.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 239-269.
  • 136. Fang, G. and Cech, T. R. Oxytricha Telomere-binding Protein: DNA-dependent Dimerization of the alpha and beta Subunits. Proc. Natl. Acad. Sci. USA 90, 6056-6060.
  • 137. Ishikawa, F., Matunis, M. J., Dreyfuss, G. and Cech, T. R. Nuclear Proteins that Bind the Pre-mRNA 3' Splice Site Sequence r(UUAG/G) and the Human Telomeric DNA Sequence d(TTAGGG)n. Mol. Cell. Biol. 13, 4301-4310.
  • 138. Zaug, A. J., McEvoy, M. M. and Cech, T. R. Self-Splicing of the Group I Intron fromAnabaena pre-tRNA Requires Base-pairing of the Exons in the Anticodon Stem. Biochemistry 32, 7946-7953.
  • 139. Herschlag, D., Eckstein, F. and Cech, T. R. Contributions of 2' Hydroxyl Groups of the RNA Substrate to Binding and Catalysis by the Tetrahymena Ribozyme. An Energetic Picture of an Active Site Composed of RNA. Biochemistry 32, 8299-8311.
  • 140. Herschlag, D., Eckstein, F. and Cech, T. R. The Importance of Being Ribose at the Cleavage Site in the Tetrahymena Ribozyme Reaction. Biochemistry 32, 8312-8321.
  • 141. Cech, T. R. Fishing for Fresh RNA Catalysts (News & Views). Nature 365, 204-205.
  • 142. Fang, G. and Cech, T. R. The beta Subunit of Oxytricha Telomere-binding Protein Promotes G-quartet Formation by Telomeric DNA. Cell 74, 875-885.
  • 143. McConnell, T. S., Cech, T. R. and Herschlag, D. Guanosine Binding to the TetrahymenaRibozyme: Thermodynamic Coupling with Oligonucleotide Binding. Proc. Natl. Acad. Sci. USA90, 8362-8366.
  • 144. Fang, G. and Cech, T. R. Characterization of a G-quartet Formation Reaction Promoted by the beta Subunit of Oxytricha Telomere-binding Protein. Biochemistry 32, 11646-11657.
  • 145. Sullenger, B. A. and Cech, T. R. Tethering Ribozymes to a Retroviral Packaging Signal for Destruction of Viral RNA. Science 262, 1566-1569.
  • 146. Strobel, S. A. and Cech, T. R. Tertiary Interactions with the Internal Guide Sequence Mediate Docking of the P1 Helix into the Catalytic Core of the Tetrahymena Ribozyme. Biochemistry 32, 13593-13604.
  • 147. Willis, M. C., Hicke, B. J., Uhlenbeck, O. C., Cech, T. R. and Koch, T. H. Photocrosslinking of 5-Iodouracil-Substituted RNA and DNA to Proteins. Science 262, 1255-1257.
  • 148. Cech, T. R. Efficiency and Versatility of Catalytic RNA: Implications for an RNA World.Gene 135, 33-36.

1992

  • 124. Wang, J.-F. and Cech, T. R. Tertiary Structure around the Guanosine-Binding Site of theTetrahymena Ribozyme. Science 256, 526-529.
  • 125. Piccirilli, J. A., McConnell, T. S., Zaug, A. J., Noller, H. F. and Cech, T. R. Aminoacyl Esterase Activity of the Tetrahymena Ribozyme. Science 256, 1420-1424.p>
  • 126. Pyle, A. M., Murphy, F. L. and Cech, T. R. RNA Substrate Binding Site in the Catalytic Core of the Tetrahymena Ribozyme. Nature 358, 123-128.
  • 127. Cech, T. R. Ribozyme Engineering. Curr. Opin. Struc. Biol. 2, 605-609.
  • 128. Cech, T. R., Herschlag, D., Piccirilli, J. A. and Pyle, A. M. RNA Catalysis by a Group I Ribozyme: Developing a Model for Transition State Stabilization. J. Biol. Chem. 267, 17479-17482.
  • 129. Legault, P., Herschlag, D., Celander, D. W. and Cech, T. R. Mutations at the Guanosine-binding Site of the Tetrahymena Ribozyme Also Affect Site-specific Hydrolysis. Nucleic Acids Res. 20, 6613-6619.

1991

  • 111. Celander, D. W. and Cech, T. R. Visualizing the Higher Order Folding of a Catalytic RNA Molecule. Science 251, 401-407.
  • 112. Cech, T. R. RNA Editing: World's Smallest Introns? Cell 64, 667-669.
  • 113. Woodson, S. A. and Cech, T. R. Alternative Secondary Structures in the 5' Exon Affect Both Forward and Reverse Self-Splicing of the Tetrahymena Intervening Sequence RNA. Biochemistry30, 2042-2050.
  • 114. Pyle, A. M. and Cech, T. R. Ribozyme Recognition of RNA by Tertiary Interactions with Specific Ribose 2'-OH Groups. Nature 350, 628-631.
  • 115. Zahler, A. M., Williamson, J. R., Cech, T. R. and Prescott, D. M. Inhibition of Telomerase by G-Quartet DNA Structures. Nature 350, 718-720.
  • 116. Herschlag, D., Piccirilli, J. A. and Cech, T. R. Ribozyme-catalyzed and Non-enzymatic Reactions of Phosphate Diesters: Rate Effects upon Substitution of Sulfur for a Non-bridging Phosphoryl Oxygen Atom. Biochemistry 30, 4844-4854.
  • 117. Grosshans, C. A. and Cech, T. R. A Hammerhead Ribozyme Allows Synthesis of a New Form of the Tetrahymena Ribozyme Homogeneous in Length with a 3' end Blocked for Transesterification. Nucleic Acids Res. 19, 3875-3880.
  • 118. Dávila-Aponte, J. A., Huss, V. A. R., Sogin, M. L. and Cech, T. R. A Self-splicing Group I Intron in the Nuclear Pre-rRNA of the Green Alga, Ankistrodesmus stipitatus. Nucleic Acids Res.19, 4429-4436.
  • 119. Gampel, A. and Cech, T. R. Binding of the CBP2 Protein to a Yeast Mitochondrial Group I Intron Requires the Catalytic Core of the RNA. Genes & Development 5, 1870-1880.
  • 120. Gray, J. T., Celander, D. W., Price, C. M. and Cech, T. R. Cloning and Expression of Genes for the Oxytricha Telomere-Binding Protein: Specific Subunit Interactions Involved in the Formation of the Telomeric Complex. Cell 67, 807-814.
  • 121. Young, B., Herschlag, D. and Cech, T. R. Mutations in a Nonconserved Sequence of theTetrahymena Ribozyme Increase Activity and Specificity. Cell 67, 1007-1019.
  • 122. Fang, G. and Cech, T. R. Molecular Cloning of Telomere-binding Protein Genes fromStylonychia mytilis. Nucleic Acids Res. 19, 5515-5518.
  • 123. Heuer, T. S., Chandry, P. S., Belfort, M., Celander, D. W. and Cech, T. R. Folding of Group I Introns from Bacteriophage T4 Involves Internalization of the Catalytic Core. Proc. Natl. Acad. Sci. USA 88, 11105-11109.

1990

  • 100. Celander, D. W. and Cech, T. R. Iron(II)-Ethylenediaminetetra-acetic Acid-Catalyzed Cleavage of RNA and DNA Oligonucleotides: Similar Reactivity toward Single- and Double-Stranded Forms. Biochemistry 29, 1355-1361.
  • 101. Hicke, B. J., Celander, D. W., MacDonald, G. H., Price, C. M. and Cech, T. R. Two Versions of the Gene Encoding the 41-kilodalton Subunit of the Telomere Binding Protein ofOxytricha nova. Proc. Natl. Acad. Sci. USA 87, 1481-1485.
  • 102. Herschlag, D. and Cech, T. R. DNA Cleavage Catalysed by the Ribozyme fromTetrahymena. Nature 344, 405-409.
  • 103. Latham, J. A., Zaug, A. J. and Cech, T. R. Self-Splicing and Enzymatic Cleavage of RNA by a Group I Intervening Sequence. Methods Enzymol. 181, 558-569.
  • 104. Downs, W. D. and Cech, T. R. An Ultraviolet-Inducible Adenosine-Adenosine Crosslink Reflects the Catalytic Structure of the Tetrahymena Ribozyme. Biochemistry 29, 5605-5613.
  • 105. Cech, T. R. Self-Splicing of Group I Introns. Annu. Rev. Biochem. 59, 543-568.
  • 106. Cech, T. R. Self-Splicing and Enzymatic Activity of an Intervening Sequence RNA fromTetrahymena. Les Prix Nobel 1989, (T. Frängsmyr, Ed., Almqvist & Wiksell Int., Stockholm) pp. 162-188. Reprinted in: Angew. Chemie 29, 759-768; Bioscience Reports 10, 240-261.
  • 107. Raghuraman, M. K. and Cech, T. R. Effect of Monovalent Cation-Induced Telomeric DNA Structure on the Binding of Oxytricha Telomeric Protein. Nucleic Acids Res. 18, 4543-4552.
  • 108. Pyle, A. M., McSwiggen, J. A. and Cech, T. R. Direct Measurement of Oligonucleotide Substrate Binding to Wild Type and Mutant Ribozymes from Tetrahymena. Proc. Natl. Acad. Sci. USA 87, 8187-8191.
  • 109. Herschlag, D. and Cech, T. R. Catalysis of RNA Cleavage by the Tetrahymena thermophilaRibozyme. 1. Kinetic Description of the Reaction of an RNA Substrate Complementary to the Active Site. Biochemistry 29, 10159-10171.
  • 110. Herschlag, D. and Cech, T. R. Catalysis of RNA Cleavage by the Tetrahymena thermophilaRibozyme. 2. Kinetic Description of the Reaction of an RNA Substrate that Forms a Mismatch at the Active Site. Biochemistry 29, 10172-10180.

1989

  • 85. Price, C. M. and Cech, T. R. Properties of the Telomeric DNA-Binding Protein fromOxytricha nova. Biochemistry 28, 769-774.
  • 86. Cech, T. R. RNA As An Enzyme. Biochem. Int. 18, 7-14.
  • 87. Woodson, S. A. and Cech, T. R. Reverse Self-splicing of the Tetrahymena Group I Intron: Implication for the Directionality of Splicing and for Intron Transposition. Cell 57, 335-345.
  • 88. McSwiggen, J. A. and Cech, T. R. Stereochemistry of RNA Cleavage by the TetrahymenaRibozyme and Evidence that the Chemical Step is Not Rate-limiting. Science 244, 679-683.
  • 89. Cech, T. R. Self-Replication?. Nature 339, 507-508.
  • 90. Raghuraman, M. K., Dunn, C. J., Hicke, B. J. and Cech, T. R. Oxytricha Telomeric Nucleoprotein Complexes Reconstituted with Synthetic DNA. Nucleic Acids Res. 17, 4235-4253.
  • 91. Latham, J. A. and Cech, T. R. Defining the Inside and Outside of a Catalytic RNA Molecule.Science 245, 276-282.
  • 92. Barfod, E. T. and Cech, T. R. The Conserved U.G Pair in the 5' Splice Site Duplex of a Group I Intron Is Required in the First but Not the Second Step of Self-Splicing. Molec. Cell. Biol. 9, 3657-3666.
  • 93. Grosshans, C. A. and Cech, T. R. Metal Ion Requirements for Sequence-Specific Endoribo-nuclease Activity of the Tetrahymena Ribozyme. Biochemistry 28, 6888-6894.
  • 94. Yarus, M., Levine, J., Morin, G. B. and Cech, T. R. A Tetrahymena Intron Nucleotide Connected to the GTP/Arginine Site. Nucleic Acids Res. 17, 6969-6981.
  • 95. Flor, P., Flanegan, J. B. and Cech, T. R. A Conserved Base-pair within Helix P4 of theTetrahymena Ribozyme Helps to Form the Tertiary Structure Required for Self-Splicing. EMBO J.8, 3391-3399.
  • 96. Raghuraman, M. K. and Cech, T. R. Assembly and Self-association of Oxytricha Telomeric Nucleoprotein Complexes. Cell 59, 719-728 (1989).
  • 97. Murphy, F. L. and Cech, T. R. Alteration of Substrate Specificity for the Endoribonucleolytic Cleavage of RNA by the Tetrahymena Ribozyme. Proc. Natl. Acad. Sci. USA 86, 9218-9222.
  • 98. Young, B. and Cech, T. R. Specificity for 3',5'-Linked Substrates in RNA-Catalyzed RNA Polymerization. J. Molec. Evol. 29, 480-485.
  • 99. Williamson, J., Raghuraman, M. K. and Cech, T. R. Monovalent Cation-induced Structure of Telomeric DNA: The G-quartet Model. Cell 59, 871-880.

1988

  • 73. Morin, G. B. and Cech, T. R. Phylogenetic Relationships and Altered Genome Structures among Tetrahymena Mitochondrial DNAs. Nucleic Acids Res. 16, 327-346.
  • 74. Cech, T. R. Biologic Catalysis by RNA. In The Harvey Lectures, Series 82 (Alan R. Liss, Inc., New York) pp. 123-144.
  • 75. Morin, G. B. and Cech, T. R. Mitochondrial Telomeres: Surprising Diversity of Repeated Telomeric DNA Sequences among Six Species of Tetrahymena. Cell 52, 367-374.
  • 76. Been, M. D. and Cech, T. R. RNA as an RNA Polymerase: Net Elongation of an RNA Primer Catalyzed by the Tetrahymena Ribozyme. Science 239, 1412-1416.
  • 77. Been, M. D., Barfod, E. T., Burke, J. M., Price, J. V., Tanner, N. K., Zaug, A. J. and Cech, T. R. Structures Involved in Tetrahymena rRNA Self-Splicing and RNA Enzyme Activity. Cold Spring Harbor Symp. Quant. Biol., Vol. LII, 147-157.
  • 78. Cech, T. R. G-Strings at Chromosome Ends (News & Views). Nature 332, 777-778.
  • 79. Barfod, E. T. and Cech, T. R. Deletion of Nonconserved Helices Near the 3' end of the rRNA Intron of Tetrahymena thermophila Alters Self-Splicing But Not Core Catalytic Activity. Genes Dev. 2, 652-663.
  • 80. Morin, G. B. and Cech, T. R. Telomeric Repeats of Tetrahymena malaccensis Mitochondrial DNA: A Multimodal Distribution That Fluctuates Erratically During Growth. Molec. Cell. Biol. 8, 4450-4458.
  • 81. Price, J. V. and Cech, T. R. Determinants of the 3' Splice Site for Self-Splicing of theTetrahymena pre-rRNA. Genes Dev. 2, 1439-1447.
  • 82. Cech, T. R. Ribozymes and Their Medical Implications. J. Am. Med. Assn. 260, 3030-3034.
  • 83. Zaug, A. J., Grosshans, C. A. and Cech, T. R. Sequence-Specific Endoribonuclease Activity of the Tetrahymena Ribozyme: Enhanced Cleavage of Certain Oligonucleotide Substrates that Form Mismatched Ribozyme-Substrate Complexes. Biochemistry 27, 8924-8931.
  • 84. Cech, T. R. Conserved Sequences and Structures of Group I Introns: Building an Active Site for RNA Catalysis. Gene 73, 259-271.

1987

  • 64. Dinter-Gottlieb, G. and Cech, T. R. Viroids Contain Sequences Characteristic of Group I Introns. In Transcriptional Control Mechanisms, UCLA Symp. Molec. Cell. Biol. (D. Granner, M. G. Rosenfeld, S. Chang, eds., Alan R. Liss, N.Y.) Vol. 52, 171-180.
  • 65. Cech, T. R. The Chemistry of Self-Splicing RNA and RNA Enzymes. Science 236, 1532-1539.
  • 66. Tanner, N. K. and Cech, T. R. Guanosine Binding Required for Cyclization of the Self-Splicing Intervening Sequence Ribonucleic Acid from Tetrahymena thermophila. Biochemistry 26, 3330-3340.
  • 67. Price, J. V., Engberg, J. and Cech, T. R. 5' Exon Requirement for Self-splicing of theTetrahymena thermophila Pre-ribosomal RNA and Identification of a Cryptic 5' Splice Site in the 3' Exon. J. Mol. Biol. 196, 49-60.
  • 68. Been, M. D. and Cech, T. R. Selection of Circularization Sites in a Group I IVS RNA Requires Mul-tiple Alignments of an Internal Template-like Sequence. Cell 50, 951-961.
  • 69. Burke, J. M., Belfort, M., Cech, T. R., Davies, R. W., Schweyen, R. J., Shub, D. A., Szostak, J. W. and Tabak, H. F. Structural Conventions for Group I Introns. Nucleic Acids Res. 15, 7217-7221.
  • 70. Price, C. M. and Cech, T. R. Telomeric DNA-Protein Interactions of Oxytricha Macronuclear DNA. Genes Dev. 1, 783-793.
  • 71. Cech, T. R., Zaug, A. J. and Been, M. D. Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena. In Molecular Biology of RNA: New Perspectives (M. Inouye and B. S. Dudock, eds., Academic Press, New York) pp. 37-44.
  • 72. Kim, S.-H. and Cech, T. R. Three-dimensional Model of the Active Site of the TetrahymenaRibozyme. Proc. Natl. Acad. Sci. USA 84, 8788-8792.

1986

  • 48. Cech, T. R. The Generality of Self-Splicing RNA: Relationship to Nuclear mRNA Splicing. Cell44, 207-210.
  • 49. Zaug, A. J. and Cech, T. R. The Intervening Sequence RNA of Tetrahymena Is an Enzyme.Science 231, 470-475.
  • 50. Burke, J. M., Irvine, K. D., Kaneko, K. J., Kerker, B. J., Oettgen, A. B., Tierney, W. M., Williamson, C. L., Zaug, A. J. and Cech, T. R. Role of Conserved Sequence Elements 9L and 2 in Self-Splicing of the Tetrahymena Ribosomal RNA Precursor. Cell 45, 167-176.
  • 51. Inoue, T., Sullivan, F. X. and Cech, T. R. New Reactions of the Ribosomal RNA Precursor ofTetrahymena and the Mechanism of Self-splicing. J. Mol. Biol. 189, 143-165.
  • 52. Cech, T. R. and Bass, B. L. Biological Catalysis by RNA. Ann. Rev. Biochem. 55, 599-629.
  • 53. Cech, T. R. A Model for the RNA-catalyzed Replication of RNA. Proc. Natl. Acad. Sci. USA83, 4360-4363.
  • 54. Garriga, G., Lambowitz, A. M., Inoue, T. and Cech, T. R. Mechanism of Recognition of the 5' Splice Site in Self-splicing Group I Introns. Nature 322, 86-89.
  • 55. Bass, B. L. and Cech, T. R. Ribozyme Inhibitors: Deoxyguanosine and Dideoxyguanosine Are Competitive Inhibitors of Self-Splicing of the Tetrahymena Ribosomal Ribonucleic Acid Precursor.Biochemistry 25, 4473-4477.
  • 56. Zaug, A. J. and Cech, T. R. The Tetrahymena Intervening Sequence Ribonucleic Acid Enzyme Is a Phosphotransferase and an Acid Phosphatase. Biochemistry 25, 4478-4482.
  • 57. Cech, T. R. Ribosomal RNA Gene Expression in Tetrahymena: Transcription and RNA Splicing. In The Molecular Biology of Ciliated Protozoa (J. G. Gall, Ed., Academic Press: New York) pp. 203-225.
  • 58. Morin, G. B. and Cech, T. R. The Telomeres of the Linear Mitochondrial DNA ofTetrahymena thermophila Consist of 53 bp Tandem Repeats. Cell 46, 873-883.
  • 59. Cech, T. R. Mechanism of Self-Splicing of the Ribosomal RNA Precursor of Tetrahymena. InGenetic Chemistry: The Molecular Basis of Heredity, Robert A. Welch Foundation Confer-ences on Chemical Research, Vol. XXIX, pp. 345-350.
  • 60. Cech, T. R., Sullivan, F. X., Inoue, T., Burke, J. M., Been, M. D., Tanner, N. K. and Zaug, A. J. Conformational Dynamics Involved in RNA Self-Splicing. In Structure and Dynamics of RNA(eds. van Knippenberg, P. H. and Hilbers, C. W., Plenum: New York) NATO ASI Series A, vol. 110, pp. 303-308.
  • 61. Cech, T. R. RNA as an Enzyme. Scientific American 255, 64-75.
  • 62. Been, M. D. and Cech, T. R. One Binding Site Determines Sequence Specificity ofTetrahymena Pre-rRNA Self-Splicing, Trans-Splicing and RNA Enzyme Activity. Cell 47, 207-216.
  • 63. Zaug, A. J., Been, M. D. and Cech, T. R. The Tetrahymena Ribozyme Acts like an RNA Restriction Endonuclease. Nature 324, 429-433.

1985

  • 37. Inoue, T. and Cech, T. R. Secondary Structure of the Circular Form of the TetrahymenaRibosomal RNA Intervening Sequence: A Technique for RNA Structure Analysis Using Chemical Probes and Reverse Transcriptase. Proc. Natl. Acad. Sci. USA 82, 648-652.
  • 38. Cech, T. R. Self-Splicing RNA: Implications for Evolution. In Genome Evolution inProkaryotes and Eukaryotes, International Review of Cytology 93, 3-22.
  • 39. Price, J. V., Kieft, G. L., Kent, J. R., Sievers, E. L. and Cech, T. R. Sequence Requirements for Self-Splicing of the Tetrahymena thermophila Pre-Ribosomal RNA. Nucleic Acids Res. 13, 1871-1889.
  • 40. Price, J. V. and Cech, T. R. Coupling of Tetrahymena Ribosomal RNA Splicing to beta-Galactosidase Expression in Escherichia coli. Science 228, 719-722.
  • 41. Zaug, A. J. and Cech, T. R. Oligomerization of Intervening Sequence RNA Molecules in the Absence of Proteins. Science 229, 1060-1064.
  • 42. Sullivan, F. X. and Cech, T. R. Reversibility of Cyclization of the Tetrahymena rRNA Intervening Sequence: Implication for the Mechanism of Splice-Site Choice. Cell 42, 639-648.
  • 43. Tanner, N. K. and Cech, T. R. Self-Catalyzed Cyclization of the Intervening Sequence RNA ofTetrahymena: Inhibition by Intercalating Dyes. Nucleic Acids Res. 13, 7741-7758.
  • 44. Tanner, N. K. and Cech, T. R. Self-Catalyzed Cyclization of the Intervening Sequence RNA ofTetrahymena: Inhibition by Methidiumpropyl EDTA and Localization of the Major Dye Binding Sites. Nucleic Acids Res. 13, 7759-7779.
  • 45. Zaug, A. J., Kent, J. R. and Cech, T. R. Reactions of the Intervening Sequence of theTetrahymena Ribosomal Ribonucleic Acid Precursor: pH Dependence of Cyclization and Site-Specific Hydrolysis. Biochemistry 24, 6211-6218.
  • 46. Been, M. D. and Cech, T. R. Sites of Circularization of the Tetrahymena rRNA IVS are Determined by Sequence and Influenced by Position and Secondary Structure. Nucleic Acids Res.13, 8389-8408.
  • 47. Inoue, T., Sullivan, F. X. and Cech, T. R. Intermolecular Exon Ligation of the rRNA ofTetrahymena: Oligonucleotides Can Function as 5' Exons. Cell 43, 431-437.

1984

  • 32. Pardue, M. L., Fostel, J. M. and Cech, T. R. DNA-Protein Interactions in the Drosophila virilis Mitochondrial Chromosome. Nucleic Acids Res. 12, 1991-1999.
  • 33. Palen, T. E. and Cech, T. R. Chromatin Structure at the Replication Origins and Transcription-Initiation Regions of the Ribosomal RNA Genes of Tetrahymena. Cell 36, 933-942.
  • 34. Bass, B. L. and Cech, T. R. Specific Interaction Between the Self-Splicing RNA ofTetrahymena and its Guanosine Substrate: Implications for Biological Catalysis by RNA. Nature308, 820-826.
  • 35. Zaug, A. J., Kent, J. R. and Cech, T. R. A Labile Phosphodiester Bond at the Ligation Junction in a Circular Intervening Sequence RNA. Science 224, 574-578.
  • 36. Gottschling, D. E. and Cech, T. R. Chromatin Structure of the Molecular Ends of OxytrichaMacronuclear DNA: Phased Nucleosomes and a Telomeric Complex. Cell 38, 501-510.

1983

  • 25. Zaug, A. J., Grabowski, P. J. and Cech, T. R. Autocatalytic Cyclization of an Excised Intervening Sequence RNA is a Cleavage-Ligation Reaction. Nature 301, 578-583.
  • 26. Palen, T. E. and Cech, T. R. Transcribed and Non-Transcribed Regions of TetrahymenaRibosomal Gene Chromatin Have Different Accessibilities to Micrococcal Nuclease. Nucleic Acids Res. 11, 2077-2091.
  • 27. Gottschling, D. E., Palen, T. E. and Cech, T. R. Different Nucleosome Spacing in Transcribed and Non-Transcribed Regions of the Ribosomal RNA Gene in Tetrahymena thermophila.Nucleic Acids Res. 11, 2093-2109.
  • 28. Brehm, S. L. and Cech, T. R. Fate of an Intervening Sequence Ribonucleic Acid: Excision and Cyclization of the Tetrahymena Ribosomal RNA Intervening Sequence in vivo. Biochemistry 22, 2390-2397.
  • 29. Cech, T. R., Tanner, N. K., Tinoco, I., Jr., Weir, B. R., Zuker, M. and Perlman, P. S. Secondary Structure of the Tetrahymena Ribosomal RNA Intervening Sequence: Structural Homology with Fungal Mitochondrial Intervening Sequences. Proc. Nat. Acad. Sci. USA 80, 3903-3907.
  • 30. Cech, T. R. RNA Splicing: Three Themes with Variations (Minireview). Cell 34, 713-716.
  • 31. Grabowski, P. J., Brehm, S. L., Zaug, A. J., Kruger, K. and Cech, T. R. Self-Splicing of the Ribosomal RNA Precursor of Tetrahymena. In Gene Expression (O. J. Hammer and M. J. Rosenberg, eds.; Alan R. Liss, N.Y.) Vol. 8, 327-342.

1982

  • 21. Zaug, A. J. and Cech, T. R. The Intervening Sequence Excised from the Ribosomal RNA Precursor of Tetrahymena Contains a 5-Terminal Guanosine Residue not Encoded by the DNA.Nucleic Acids Res. 10, 2823-2838.
  • 22. Cech, T. R., Zaug, A. J., Grabowski, P. J. and Brehm, S. L. Transcription and Splicing of the Ribosomal RNA Precursor of Tetrahymena. In The Cell Nucleus (H. Busch and L. Rothblum, eds.; Academic Press, NY) Vol. X, pp. 171-204.
  • 23. Cech, T., Zaug, A., Grabowski, P. and Brehm, S. Processing of Ribosomal RNA. In RNA Processing (S. J. Flint, ed.) Fed. Proc. 41, 2781-2789.
  • 24. Kruger, K., Grabowski, P. J., Zaug, A. J., Sands, J., Gottschling, D. E. and Cech, T. R. Self-Splicing RNA: Autoexcision and Autocyclization of the Ribosomal RNA Intervening Sequence ofTetrahymena. Cell 31, 147-157.

1981

  • 17. Cech, T. R. Alkaline Gel Electrophoresis of Deoxyribonucleic Acid Photoreacted with Trimethylpsoralen: Rapid and Sensitive Detection of Interstrand Cross-Links. Biochemistry 20, 1431-1437.
  • 18. Grabowski, P. J., Zaug, A. J. and Cech, T. R. The Intervening Sequence of the Ribosomal RNA Precursor is Converted to a Circular RNA in Isolated Nuclei of Tetrahymena. Cell 23, 467-476.
  • 19. Cech, T. R. and Brehm, S. L. Replication of the Extrachromosomal Ribosomal RNA Genes ofTetrahymena thermophila. Nucleic Acids Res. 9, 3531-3543.
  • 20. Cech, T. R., Zaug, A. J. and Grabowski, P. J. In vitro Splicing of the Ribosomal RNA Precursor of Tetrahymena: Involvement of a Guanosine Nucleotide in the Excision of the Intervening Sequence. Cell 27, 487-496.

1980

  • 14. Zaug, A. J. and Cech, T. R. In vitro Splicing of the Ribosomal RNA Precursor in Nuclei ofTetrahymena. Cell 19, 331-338.
  • 15. Cech, T. R. and Karrer, K. M. Chromatin Structure of the Ribosomal RNA Genes ofTetrahymena as Analyzed by in vivo Trimethylpsoralen Crosslinking. J. Mol. Biol. 136, 395-416.
  • 16. Potter, D. A., Fostel, J. M., Berninger, M., Pardue, M. L. and Cech, T. R. DNA-protein Interactions in the Drosophila melanogaster Mitochondrial Genome as Deduced from Trimethylpsoralen Crosslinking Patterns. Proc. Natl. Acad. Sci. USA 77, 4118-4122.

1979

  • 12. Cech, T., Pathak, M. A. and Biswas, R. K. An Electron Microscopic Study of the Photochemical Cross-linking of DNA in Guinea Pig Epidermis by Psoralen Derivatives. Biochim. Biophys. Acta 562, 342-360.
  • 13. Cech, T. and Rio, D. C. Localization of Transcribed Regions on the Extrachromosomal Ribosomal RNA Genes of Tetrahymena thermophila by R-loop Mapping. Proc. Natl. Acad. Sci. USA 76, 5051-5055.

1978

  • 10. Cech, T., Potter, D. and Pardue, M. L. Chromatin Structure in Living Cells. Cold Spring Harbor Symp. Quant. Biol. 42, 191-198.
  • 11. Berninger, M., Cech, T. R., Fostel, J., Potter, D., Scott, M. and Pardue, M. L. The Structure and Function of the Mitochondrial DNA of Drosophila melanogaster. Alfred Benzon Symp. (ed. by J. Engberg, H. Klenow, and V. Leick), Vol. 13.

1977

  • 8. Cech, T. and Pardue, M.L. Crosslinking of DNA with Trimethylpsoralen is a Probe for Chromatin Structure. Cell 11, 631-640.
  • 9. Cech, T., Potter, D. and Pardue, M. L. Electron Microscopy of DNA Crosslinked with Trimethylpsoralen: A Probe for Chromatin Structure. Biochemistry 16, 5313-5321.

1976

  • 4. Cech, T. R. and Hearst, J. E. Organization of Highly Repeated Sequences in Mouse Main-band DNA. J. Mol. Biol. 100, 227-256.
  • 5. Cech, T. R., Wiesehahn, G. and Hearst, J. E. Partial Denaturation of Mouse DNA in Preparative CsCl Density Gradients at Alkaline pH. Biochemistry 15, 1865-1873.
  • 6. Wiesehahn, G., Cech, T. R. and Hearst, J. E. A Study of DNA Denaturation in the Ultracentrifuge. Biopolymers 15, 1591-1613.
  • 7. Cech, T. R. and Pardue, M. L. Electron Microscopy of DNA Crosslinked with Trimethylpsoralen: A Test of the Secondary Structure of Eukaryotic Inverted Repeat Sequences.Proc. Natl. Acad. Sci. USA 73, 2644-2648.

1975

  • 3. Cech, T. R. and Hearst, J. E. An Electron Microscopic Study of Mouse Foldback DNA. Cell 5, 429-446.

1974

  • 2. Hearst, J. E., Cech, T. R., Marx, K. A., Rosenfeld, A. and Allen, J. R. Characterization of the Rapidly Renaturing Sequences in the Main CsCl Density Bands of Drosophila, Mouse, and Human DNA. Cold Spring Harbor Symp. Quant. Biol. 38, 329-339.

1973

  • 1. Cech, T. R., Rosenfeld, A. and Hearst, J. E. Characterization of the Most Rapidly Renaturing Sequences in Mouse Main-band DNA. J. Mol. Biol. 81, 299-325.